放线菌密苏里变种中独特的 σ/反-σ 系统。
A unique sigma/anti-sigma system in the actinomycete Actinoplanes missouriensis.
机构信息
Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Graduate School of Infection Control Sciences, Kitasato University, Minato-ku, Tokyo, Japan.
出版信息
Nat Commun. 2023 Dec 20;14(1):8483. doi: 10.1038/s41467-023-44291-y.
Bacteria of the genus Actinoplanes form sporangia that contain dormant sporangiospores which, upon contact with water, release motile spores (zoospores) through a process called sporangium dehiscence. Here, we set out to study the molecular mechanisms behind sporangium dehiscence in Actinoplanes missouriensis and discover a sigma/anti-sigma system with unique features. Protein σ contains a functional sigma factor domain and an anti-sigma factor antagonist domain, while protein SipA contains an anti-sigma factor domain and an anti-sigma factor antagonist domain. Remarkably, the two proteins interact with each other via the anti-sigma factor antagonist domain of σ and the anti-sigma factor domain of SipA. Although it remains unclear whether the SipA/σ system plays direct roles in sporangium dehiscence, the system seems to modulate oxidative stress responses in zoospores. In addition, we identify a two-component regulatory system (RsdK-RsdR) that represses initiation of sporangium dehiscence.
游动放线菌属的细菌形成包含休眠孢子囊的孢子囊，当与水接触时，通过称为孢子囊开裂的过程释放游动孢子（游动孢子）。在这里，我们着手研究游动放线菌孢子囊开裂的分子机制，并发现了一个具有独特特征的 sigma/抗 sigma 系统。蛋白 σ 包含一个功能 sigma 因子结构域和一个抗 sigma 因子拮抗结构域，而蛋白 SipA 包含一个抗 sigma 因子结构域和一个抗 sigma 因子拮抗结构域。值得注意的是，这两种蛋白质通过 σ 的抗 sigma 因子拮抗结构域和 SipA 的抗 sigma 因子结构域相互作用。虽然尚不清楚 SipA/σ 系统是否直接参与孢子囊开裂，但该系统似乎调节游动孢子的氧化应激反应。此外，我们鉴定了一个双组分调节系统（RsdK-RsdR），它抑制孢子囊开裂的起始。
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