二乙基亚硝胺诱导小鼠肝癌发生。

Diethylnitrosamine Induction of Hepatocarcinogenesis in Mice.

机构信息

Sorbonne Université, Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche de Saint Antoine (CRSA), Paris, France.

Département de Virologie, Assistance Publique - Hôpitaux de Paris (AP-HP), Sorbonne Université, GHU Paris-Est, Paris, France.

出版信息

Methods Mol Biol. 2024;2769:15-25. doi: 10.1007/978-1-0716-3694-7_2.

DOI:10.1007/978-1-0716-3694-7_2

PMID:38315386

Abstract

Diethylnitrosamine (DEN) is a chemical hepatocarcinogenic agent that triggers a large array of oncogenic mutations after a single injection. Initiated hepatocytes subsequently undergo clonal expansion within a proliferative environment, rendering the DEN model a comprehensive carcinogen. In rodent studies, DEN finds extensive utility in experimental liver cancer research, mimicking several aspects of human hepatocellular carcinoma (HCC), including angiogenesis, metabolic reprogramming, immune exhaustion, and the ability to metastasize. Beyond the wealth of scientific insights gleaned from this model, the objective of this chapter is to review morphological, genomic, and immunological characteristics associated to DEN-induced HCC. Furthermore, this chapter provides a detailed procedural guide to effectively induce hepatocarcinogenesis in mice through a single intraperitoneal injection of DEN.

摘要

二乙基亚硝胺（DEN）是一种化学性肝致癌剂，单次注射后会引发大量致癌突变。起始肝细胞随后在增殖环境中经历克隆扩张，使 DEN 模型成为一种全面的致癌剂。在啮齿动物研究中，DEN 在实验性肝癌研究中得到了广泛应用，可模拟人类肝细胞癌（HCC）的多个方面，包括血管生成、代谢重编程、免疫衰竭和转移能力。除了从该模型中获得的大量科学见解外，本章的目的还在于综述 DEN 诱导的 HCC 相关的形态学、基因组和免疫学特征。此外，本章还提供了一种详细的程序性指南，通过单次腹腔注射 DEN 有效地在小鼠中诱导肝癌发生。