Metabolic perturbation of by introducing NADP-dependent glyceraldehyde 3-phosphate dehydrogenase.

The available resources of represent a valuable repository of bioactive natural products that warrant exploration. is primarily utilized in the industrial synthesis of ε-poly-L-lysine (ε-PL). In this study, the NADP-dependent glyceraldehyde 3-phosphate dehydrogenase (GapN) from was heterologously expressed in CICC11022, leading to elevated intracellular NADPH levels and reduced NADH and ATP concentrations. The resulting perturbation of metabolism was comprehensively analyzed using transcriptomic and metabolomic methodologies. A decrease in production of ε-PL was observed. The expression of significantly impacted on 23 gene clusters responsible for the biosynthesis of secondary metabolites. A comprehensive analysis revealed a total of 21 metabolites exhibiting elevated levels both intracellularly and extracellularly in the expressing strain compared to those in the control strain. These findings underscore the potential of to generate diverse bioactive natural products, thus offering valuable insights for the utilization of known resources through genetic manipulation.