Genome-Wide Identification and Expression Analysis of the Gene Family in under Salt Stress.

The U-box protein family of ubiquitin ligases is important in the biological processes of plant growth, development, and biotic and abiotic stress responses. Plants in the genus are recognized as excellent warm-season turfgrass species with drought, wear and salt tolerance. In this study, we conducted the genome-wide identification of () genes in based on U-box domain searching. In total, 71 genes were identified, and a protein tree was constructed of AtPUBs, OsPUBs, and ZjPUBs, clustered into five groups. The gene structures, characteristics, -elements and protein interaction prediction network were analyzed. There were mainly ABRE, ERE, MYB and MYC -elements distributed in the promoter regions of . were predicted to interact with PDR1 and EXO70B1, related to the abscisic acid signaling pathway. To better understand the roles of under salt stress, the expression levels of 18 under salt stress were detected using transcriptome data and qRT-PCR analysis, revealing that 16 were upregulated in the roots under salt treatment. This indicates that might participate in the salt stress response. This research provides insight into the gene family and may support the genetic improvement in the molecular breeding of salt-tolerant zoysiagrass varieties.