Identification and biological characteristics of TN-47 isolated from .

With the widespread use of antibiotics, the incidence of antibiotic resistance in microorganisms has increased. is a trunk borer of pine trees. This study aimed to investigate the antimicrobial and biological characteristics of TN-47 (PP411196), isolated from the gastrointestinal tract of in Jilin Province, PR China. Among 13 isolates obtained from the insects, five were preliminarily screened for antimicrobial activity. TN-47, which exhibited the strongest antimicrobial activity, was identified. TN-47 possessed antimicrobial activity against USA300 and serovar Pullorum ATCC 19945. Furthermore, TN-47 was sensitive to tetracyclines, penicillins (ampicillin, carbenicillin, and piperacillin), quinolones and nitrofuran antibiotics, and resistant to certain beta-lactam antibiotics (oxacillin, cefradine and cephalexin), macrolide antibiotics, sulfonamides and aminoglycosides. TN-47 could tolerate low pH and pepsin-rich conditions in the stomach and grow in the presence of bile acids. TN-47 retained its strong auto-aggregating ability and hydrophobicity. This strain did not exhibit any haemolytic activity. These results indicate that TN-47 has potential as a probiotic. This study provides a theoretical foundation for the future applications of TN-47 and its secondary metabolites in animal nutrition and feed.