一种用于检测次氯酸的香豆素衍生荧光探针的研制及其在细胞和斑马鱼中的应用。
Development of a Coumarin-derived Fluorescent Probe for Detection of HOCl and its Application in Cells and Zebrafish.
作者信息
Chen Xin, Chen Jie, Wang Shanshan, Yu Shihua, Liu Zhigang, Zeng Xiaodan
机构信息
School of Chemical and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin, People's Republic of China.
Center of Characterization and Analysis, Jilin Institute of Chemical Technology, Jilin, People's Republic of China.
出版信息
J Fluoresc. 2025 May;35(5):2837-2844. doi: 10.1007/s10895-024-03642-8. Epub 2024 Apr 22.
We have prepared a simple, universal and efficient coumarin-derived fluorescent probe (XDS1) to detecting HOCl. The experimental findings revealed that the introduction of HOCl produced an obvious quenching effect on the probe with high selectivity and sensitivity. The calculated limit of detection (LOD) was as low as 0.02 μM. Furthermore, an impressive response time of less than 10 s was observed when XDS1 detecting HOCl. Importantly, the probe XDS1 exhibited negligible cytotoxicity, thereby facilitating its application for imaging HOCl within biological environment. The probe XDS1 had been successfully used for specific detection in cells.
我们制备了一种简单、通用且高效的香豆素衍生荧光探针（XDS1）用于检测次氯酸（HOCl）。实验结果表明，次氯酸的引入对该探针产生了明显的猝灭效应，具有高选择性和灵敏度。计算得到的检测限（LOD）低至0.02 μM。此外，当XDS1检测次氯酸时，观察到了令人印象深刻的小于10秒的响应时间。重要的是，探针XDS1表现出可忽略不计的细胞毒性，从而便于其在生物环境中用于次氯酸成像。探针XDS1已成功用于细胞中的特异性检测。
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