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深海微生物和基因工程菌株靶向分离优势的探索。

Exploration of the advantages of targeted isolation of deep-sea microorganisms and genetically engineered strains.

机构信息

School of Civil and Resource Engineering, University of Science and Technology Beijing, Beijing, 100083, China.

State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China.

出版信息

World J Microbiol Biotechnol. 2024 Nov 2;40(12):372. doi: 10.1007/s11274-024-04177-7.

Abstract

Oil, mineral processing and environmental restoration can be dangerous processes. Attempts are often made to apply microorganisms to reduce the risks, but the adaptability of terrestrial organisms is often weak. Although genetically engineered strains can improve their environmental adaptability through targeted modification, there are problems such as metabolite accumulation, poor plasmid stability and potential pathogenicity. Screening of extremophiles from the natural environment has become an inevitable choice. The special environment in the deep sea (high pressure, low temperature, low nutrition, high salinity) is a natural place for extremophiles to grow and survive, thus screening of extremophiles from the deep sea is conducive to the green and sustainable development of industry. In this paper, the application status and problems of genetically engineered strains are reviewed based on the microorganisms needed for extreme industry. This paper focuses on the application status and advantages of deep-sea microorganisms. It is found that their advantages are strong adaptability, stable gene, friendly environment, simple and convenient technology (compared with genetic engineering), which has a broad industry processes application prospect. This review broadens the scope of microbial applications.

摘要

石油、矿物加工和环境修复可能是危险的过程。人们经常试图应用微生物来降低风险,但陆地生物的适应性往往较弱。虽然通过有针对性的修饰可以提高基因工程菌株的环境适应性,但仍存在代谢物积累、质粒稳定性差和潜在致病性等问题。从自然环境中筛选极端微生物已成为必然选择。深海的特殊环境(高压、低温、低营养、高盐度)是极端微生物生长和生存的天然场所,因此从深海中筛选极端微生物有利于工业的绿色可持续发展。本文基于极端工业所需的微生物,综述了基因工程菌的应用现状及存在的问题,重点介绍了深海微生物的应用现状和优势。研究发现,深海微生物具有适应性强、基因稳定、环境友好、技术简单方便(与基因工程相比)等优点,具有广阔的工业应用前景。本综述拓宽了微生物应用的范围。

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