Characterization of the Activities of Vorinostat Against .

is a globally widespread pathogen of significant veterinary and medical importance, causing abortion or congenital disease in humans and other warm-blooded animals. Nevertheless, the current treatment options are restricted and sometimes result in toxic side effects. Hence, it is essential to discover drugs that demonstrate potent anti- activity. Herein, we found that vorinostat, a pan-HDAC inhibitor, exhibited an IC value of 260.1 nM against the RH strain and a selectivity index (SI) > 800 with respect to HFF cells. Vorinostat disrupted the entire lytic cycle of in vitro. Proteome analysis indicated that vorinostat remarkably perturbed the protein expression of , and proteins involved in "DNA replication" and "membrane" were significantly dysregulated. Furthermore, we found that vorinostat significantly enhanced ROS production and induced parasite apoptosis. Importantly, vorinostat could prolong survival in a murine model. Our findings reveal that vorinostat is effective against both in vitro and in vivo, suggesting its potential as a therapeutic option for human toxoplasmosis.