从埃及坦塔市即食街头食品中分离出的多重耐药肺炎克雷伯菌的表型和基因型筛查
Phenotypic and genotypic screening of multidrug resistant Klebsiella pneumoniae isolated from ready to eat street food in Tanta, Egypt.
作者信息
Bedair Hadeer M, Emara Mohamed, Ali Shima Mahmoud, Samir Tamer M, Khalil Mahmoud A F
机构信息
Department of Microbiology and Immunology, College of Pharmaceutical sciences and drug manufacturing, Misr University for Science and technology, Cairo, 12566, Egypt.
Department of Microbiology and Immunology, Faculty of Pharmacy, Helwan University - Ain Helwan, Helwan, 11795, Egypt.
出版信息
BMC Microbiol. 2025 Feb 5;25(1):65. doi: 10.1186/s12866-025-03769-z.
BACKGROUND
Ready-To-Eat-Street-Foods (RTESF) have food safety concerns, since they are prepared with less-structured food safety guidelines in small and roadside outlets. Klebsiella pneumoniae has become a dangerous foodborne-pathogen worldwide due to its virulence and resistance profile.
OBJECTIVE
This study aimed at evaluating the potential burden of antibiotic-resistant Klebsiella pneumoniae contaminating RTESF and assessing the microbiological quality of RTESF in Egypt.
METHODS
A total of 242 RTESF (green salad) samples was collected, different media were used for isolation of different bacterial species. Klebsiella pneumoniae isolates were identified biochemically and by Gram and capsular staining then isolates were assessed for antimicrobial resistance phenotypically. The ability of biofilm formation was assessed using crystal violet and molecular characterization of ESBLs and virulence genes was done using PCR.
RESULTS
A total of 77/242(31.8%) of the recovered isolates was identified as Klebsiella pneumoniae and the resistance percentages were as follow: cefuroxime and cephradine (100%, 77/77), amoxicillin-clavulanic acid (98.7%, 76/77), while (27.3%, 21/77) of the isolates were MDR. Biofilm assay revealed that (31/77, 41/77 and 5/77) isolates were strong, moderate, and weak biofilm-producers, respectively. Among ESBLs-encoding-genes, bla was the most prevalent (71.4%) while blaand blawere equally-present (55.8%).The most prevalent virulence genes were mrkD (92.2%) followed by K2 (63.3%).
CONCLUSION
The contaminated RTESF could be a reservoir for Klebsiella pneumoniae, therefore much care must be taken during preparation and consumption to avoid dissemination of MDR Klebsiella pneumoniae leading to subsequent treatment challenges. Our finding indicating that RTESF, if not prepared under hygienic conditions, could be a source of serious Klebsiella pneumoniae infection.
背景
即食街头食品（RTESF）存在食品安全问题，因为它们是在小型路边摊点按照不太严格的食品安全准则制作的。肺炎克雷伯菌由于其毒力和耐药性特征，已成为全球一种危险的食源性病原体。
目的
本研究旨在评估污染即食街头食品的耐抗生素肺炎克雷伯菌的潜在负担，并评估埃及即食街头食品的微生物质量。
方法
共采集242份即食街头食品（蔬菜沙拉）样本，使用不同培养基分离不同细菌种类。通过生化鉴定、革兰氏染色和荚膜染色鉴定肺炎克雷伯菌分离株，然后对分离株进行表型抗菌药物敏感性评估。使用结晶紫评估生物膜形成能力，并使用聚合酶链反应（PCR）对超广谱β-内酰胺酶（ESBLs）和毒力基因进行分子特征分析。
结果
共77/242（31.8%）的分离株被鉴定为肺炎克雷伯菌，耐药率如下：头孢呋辛和头孢拉定（100%，77/77），阿莫西林-克拉维酸（98.7%，76/77），而（27.3%，21/77）的分离株为多重耐药（MDR）。生物膜检测显示，（31/77、41/77和5/77）的分离株分别为强、中、弱生物膜产生菌。在ESBLs编码基因中，bla最常见（71.
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