Cross-platform detection of microplastics in human biological tissues: Comparing spectroscopic and chromatographic approaches.

Microplastic (MP) contamination in ecosystems underscores concerns about human bioaccumulation, yet analytical challenges persist due to complex biological matrices and polymer diversity. To systematically evaluate the efficacy of complementary analytical platforms, we conducted this study to systematically evaluate Raman microscopy and pyrolysis gas chromatography-mass spectrometry (py-GC/MS) for complementary MP detection in human biological samples. Building upon prior research frameworks, 48 paired endometrial and urine samples from parturient women were analyzed under rigorously controlled protocols to minimize exogenous contamination. Raman microscopy identified six polymer types, with polytetrafluoroethylene (PTFE) and polystyrene (PS) constituting primary components across both sample types. Particle size distributions spanned 1.23-6.98 μm, exhibiting comparable mean diameters in urine (2.85 ± 1.26 μm) and endometrial samples (2.89 ± 1.40 μm). Subsequent py-GC/MS analysis revealed previously undetected polymer co-occurrences (PS, PC, PE, and PVC) in samples initially classified as single-polymer PTFE or PS via Raman spectroscopy, thereby exposing inherent disparities in method-specific sensitivity and resolution. The follow-up multi-method comparison demonstrates that Raman microscopy excels in particle-specific morphological characterization, while py-GC/MS provides polymer quantification and composite identification. Our findings underscore the necessity of integrating orthogonal analytical approaches to overcome methodological limitations and achieve comprehensive MP profiling in complex biological systems.