使用介电泳量化细胞的电生理特性。
Quantifying the Electrophysiological Properties of Cells Using Dielectrophoresis.
作者信息
Clarke Krista S P, Griffiths Oreoluwa V, Hoettges Kai, Lewis Rebecca, Labeed Fatima H
机构信息
Centre for Biomedical Engineering, School of Mechanical Engineering Sciences, University of Surrey, Guildford, Surrey, UK.
Department of Electrical Engineering and Electronics, University of Liverpool, Liverpool, UK.
出版信息
Methods Mol Biol. 2025;2908:191-209. doi: 10.1007/978-1-0716-4434-8_13.
The 3DEP Reader (DEPtech) is a commercial noninvasive, high throughput, easily adoptable benchtop microfluidic platform that utilizes the principles of dielectrophoresis to analyze cells in suspension and determine their electrical properties. This protocol describes the operation, value, and common setup of the platform to provide users with the ability to characterize three important electrophysiological parameters of approximately 20,000 cells in under 2 min. Dielectrophoresis (DEP) is an electrokinetic phenomenon which describes the movement of suspended polarizable cells, in a nonuniform electric field. The 3DEP plots the relative DEP force experienced by cells across a range of AC frequencies generating a spectrum through which membrane conductance, membrane capacitance, and cytoplasmic conductivity are derived.
3DEP Reader（DEPtech公司）是一款商业化的非侵入性、高通量、易于采用的台式微流控平台，它利用介电泳原理分析悬浮细胞并确定其电学特性。本方案描述了该平台的操作、价值和常见设置，以便为用户提供在2分钟内表征约20000个细胞的三个重要电生理参数的能力。介电泳（DEP）是一种电动现象，描述了悬浮的可极化细胞在非均匀电场中的运动。3DEP绘制了细胞在一系列交流频率下所经历的相对DEP力，生成一个频谱，通过该频谱可推导出膜电导、膜电容和细胞质电导率。
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