长链非编码RNA SH3BP5-AS1在非小细胞肺癌中的预后价值及其对肿瘤进展的调控作用

Prognostic value of LncRNA SH3BP5-AS1 in non-small cell lung cancer and its regulatory effect on tumor progression.

作者信息

Meng Cong, Zang Aihua, Du Fengcai

机构信息

Department of Radiotherapy for Tumors, The Affiliated Hospital of Qingdao University, Qingdao, 266000, Shandong, China.

Department of Ultrasound, Qingdao Municipal Hospital, Qingdao, 266011, Shandong, China.

出版信息

Discov Oncol. 2025 Jun 17;16(1):1126. doi: 10.1007/s12672-025-02906-4.

DOI:10.1007/s12672-025-02906-4

PMID:40523970

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12170978/

Abstract

OBJECTIVE

This study aimed to investigate the prognostic significance of SH3BP5-AS1 in non-small cell lung cancer (NSCLC) patients, and also to uncover its function in the progression of NSCLC.

METHODS

100 NSCLC patients were recruited in this study. SH3BP5-AS1 was measured by RT-qPCR. The prognostic significance of SH3BP5-AS1 in NSCLC progression was appraised utilizing the Kaplan-Meier and Cox regression test. CCK-8 was used to detect the proliferative viability of NSCLC cells. The migratory and invasive capabilities of NSCLC cells were evaluated by Transwell assay. The interplay between SH3BP5-AS1 and miR-424-5p was verified by luciferase reporter assay and ENCORI database. The potential target genes of miR-424-5p were inspected by means of bioinformatics analysis.

RESULTS

A reduced SH3BP5-AS1 was manifested in tissues of NSCLC patients and tumor cells. Compared with patients showing SH3BP5-AS1 low-expression, those with SH3BP5-AS1 high-expression possessed a more favorable progression-free survival time. SH3BP5-AS1 might function as an autonomous prognostic biomarker for NSCLC. Upregulation of SH3BP5-AS1 suppressed the proliferation, migration and invasion of NSCLC cells. MiR-424-5p was a downstream target miRNA of SH3BP5-AS1, and luciferase reporter assays verified that SH3BP5-AS1 and miR-424-5p interact. In NSCLC patient tissues, miR-424-5p was upregulated and it exhibited an inverse correlation with SH3BP5-AS1. Elevation of miR-424-5p neutralized the inhibitory effect of SH3BP5-AS1 overexpression on the proliferation, migration, and invasion abilities of NSCLC cells.

CONCLUSION

SH3BP5-AS1 is involved in NSCLC development by targeting its downstream target miRNA miR-424-5p, and it is a latent prognostic indicator for NSCLC.

摘要

目的

本研究旨在探讨SH3BP5-AS1在非小细胞肺癌（NSCLC）患者中的预后意义，并揭示其在NSCLC进展中的作用。

方法

本研究招募了100例NSCLC患者。通过RT-qPCR检测SH3BP5-AS1。利用Kaplan-Meier和Cox回归检验评估SH3BP5-AS1在NSCLC进展中的预后意义。采用CCK-8检测NSCLC细胞的增殖活力。通过Transwell实验评估NSCLC细胞的迁移和侵袭能力。通过荧光素酶报告基因实验和ENCORI数据库验证SH3BP5-AS1与miR-424-5p之间的相互作用。通过生物信息学分析检测miR-424-5p的潜在靶基因。

结果

NSCLC患者组织和肿瘤细胞中SH3BP5-AS1表达降低。与SH3BP5-AS1低表达患者相比，SH3BP5-AS1高表达患者的无进展生存期更有利。SH3BP5-AS1可能作为NSCLC的独立预后生物标志物。SH3BP5-AS1的上调抑制了NSCLC细胞的增殖、迁移和侵袭。MiR-424-5p是SH3BP5-AS1的下游靶miRNA，荧光素酶报告基因实验验证了SH3BP5-AS1与miR-424-5p相互作用。在NSCLC患者组织中，miR-424-5p上调，且与SH3BP5-AS1呈负相关。miR-424-5p的升高抵消了SH3BP5-AS1过表达对NSCLC细胞增殖、迁移和侵袭能力的抑制作用。