酿酒酵母中脯氨酸和精氨酸代谢汇聚途径控制中的亚细胞区室化
Subcellular compartmentation in control of converging pathways for proline and arginine metabolism in Saccharomyces cerevisiae.
作者信息
Brandriss M C, Magasanik B
出版信息
J Bacteriol. 1981 Mar;145(3):1359-64. doi: 10.1128/jb.145.3.1359-1364.1981.
Abstract
Enzymes of proline biosynthesis and proline degradation which act on the same compound, delta 1-pyrroline-5-carboxylate, are physically separated in yeast cells. The enzyme responsible for the final step in proline biosynthesis, pyrroline-5-carboxylate reductase, converts pyrroline-5-carboxylate to proline and is located in the cytoplasm. The last enzyme in the proline degradative pathway, pyrroline-5-carboxylate dehydrogenase, converts pyrroline-5-carboxylate to glutamate and is found in the particulate fraction of the cell, presumably in the mitochondrion. By subcellular compartmentation, yeast cells avoid futile cycling between proline and pyrroline-5-carboxylate.
摘要
作用于同一化合物Δ¹-吡咯啉-5-羧酸的脯氨酸生物合成酶和脯氨酸降解酶，在酵母细胞中在物理上是分开的。负责脯氨酸生物合成最后一步的酶，即吡咯啉-5-羧酸还原酶，将吡咯啉-5-羧酸转化为脯氨酸，位于细胞质中。脯氨酸降解途径中的最后一种酶，吡咯啉-5-羧酸脱氢酶，将吡咯啉-5-羧酸转化为谷氨酸，存在于细胞的颗粒部分，可能在线粒体中。通过亚细胞区室化，酵母细胞避免了脯氨酸和吡咯啉-5-羧酸之间的无效循环。
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