牛病毒性腹泻病毒RNA翻译的内部起始

Internal initiation of translation of bovine viral diarrhea virus RNA.

作者信息

Pestova T V, Hellen C U

机构信息

Morse Institute for Molecular Genetics, State University of New York Health Science Center at Brooklyn, 450 Clarkson Avenue, Brooklyn, New York, 11203, USA.

出版信息

Virology. 1999 Jun 5;258(2):249-56. doi: 10.1006/viro.1999.9741.

DOI:10.1006/viro.1999.9741

PMID:10366562

Abstract

Initiation of translation on the bovine viral diarrhea virus (BVDV) internal ribosomal entry site (IRES) was reconstituted in vitro from purified translation components to the stage of 48S ribosomal initiation complex formation. Ribosomal binding and positioning on this mRNA to form a 48S complex did not require the initiation factors eIF4A, eIF4B, or eIF4F, and translation of this mRNA was resistant to inhibition by a trans-dominant eIF4A mutant that inhibited cap-mediated initiation of translation. The BVDV IRES contains elements that are bound independently by ribosomal 40S subunits and by eukaryotic initiation factor (eIF) 3, as well as determinants that mediate direct attachment of 43S ribosomal complexes to the initiation codon.

摘要

利用纯化的翻译组分在体外将牛病毒性腹泻病毒（BVDV）内部核糖体进入位点（IRES）的翻译起始过程重建至48S核糖体起始复合物形成阶段。核糖体与该mRNA结合并定位以形成48S复合物并不需要起始因子eIF4A、eIF4B或eIF4F，并且该mRNA的翻译对抑制帽依赖性翻译起始的反式显性eIF4A突变体的抑制作用具有抗性。BVDV IRES包含可被核糖体40S亚基和真核起始因子（eIF）3独立结合的元件，以及介导43S核糖体复合物直接附着于起始密码子的决定因素。