Toll样受体2（TLR2）和Toll样受体4（TLR4）在识别革兰氏阴性菌和革兰氏阳性菌细胞壁成分中的不同作用

Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components.

作者信息

Takeuchi O, Hoshino K, Kawai T, Sanjo H, Takada H, Ogawa T, Takeda K, Akira S

机构信息

Department of Host Defense, Research Institute for Microbial Diseases, Osaka University, Japan.

出版信息

Immunity. 1999 Oct;11(4):443-51. doi: 10.1016/s1074-7613(00)80119-3.

DOI:10.1016/s1074-7613(00)80119-3

PMID:10549626

Abstract

Toll-like receptor (TLR) 2 and TLR4 are implicated in the recognition of various bacterial cell wall components, such as lipopolysaccharide (LPS). To investigate in vivo roles of TLR2, we generated TLR2-deficient mice. In contrast to LPS unresponsiveness in TLR4-deficient mice, TLR2-deficient mice responded to LPS to the same extent as wild-type mice. TLR2-deficient macrophages were hyporesponsive to several Gram-positive bacterial cell walls as well as Staphylococcus aureus peptidoglycan. TLR4-deficient macrophages lacked the response to Gram-positive lipoteichoic acids. These results demonstrate that TLR2 and TLR4 recognize different bacterial cell wall components in vivo and TLR2 plays a major role in Gram-positive bacterial recognition.

摘要

Toll样受体（TLR）2和TLR4参与识别各种细菌细胞壁成分，如脂多糖（LPS）。为了研究TLR2在体内的作用，我们培育出了TLR2缺陷型小鼠。与TLR4缺陷型小鼠对LPS无反应不同，TLR2缺陷型小鼠对LPS的反应程度与野生型小鼠相同。TLR2缺陷型巨噬细胞对几种革兰氏阳性菌细胞壁以及金黄色葡萄球菌肽聚糖反应低下。TLR4缺陷型巨噬细胞对革兰氏阳性菌脂磷壁酸缺乏反应。这些结果表明，TLR2和TLR4在体内识别不同的细菌细胞壁成分，且TLR2在革兰氏阳性菌识别中起主要作用。