抗原性、表型和分子特征鉴定证实了从三只鹿科动物中分离出的奥氏巴贝斯虫。

Antigenic, phenotypic and molecular characterization confirms Babesia odocoilei isolated from three cervids.

作者信息

Holman P J, Madeley J, Craig T M, Allsopp B A, Allsopp M T, Petrini K R, Waghela S D, Wagner G G

机构信息

Department of Veterinary Pathobiology, Texas Veterinary Medical Center, Texas A&M University, College Station 77843-4467, USA.

出版信息

J Wildl Dis. 2000 Jul;36(3):518-30. doi: 10.7589/0090-3558-36.3.518.

DOI:10.7589/0090-3558-36.3.518

PMID:10941738

Abstract

Babesia isolates from an elk (Cervus elaphus canadensis) and a caribou (Rangifer tarandus caribou) with fatal infections were compared to Babesia odocoilei (Engeling isolate) from white-tailed deer (Odocoileus virginianus) by experimental infection, serologic, and small subunit ribosomal RNA (SSU rRNA) gene sequence analysis studies. Both the indirect fluorescent antibody test and immunoprecipitation assays demonstrated antigenic variation among the isolates. Experimental infection studies showed no clinical differences among the isolates. Nucleotide sequence analysis showed that the elk and caribou Babesia sp. isolates possessed SSU rRNA genes with identical sequences to that of B. odocoilei. A phylogenetic tree constructed from SSU rRNA gene sequences shows that B. odocoilei is most closely related to Babesia divergens, both of which branch together in the true babesia clade.

摘要

通过实验感染、血清学和小亚基核糖体RNA（SSU rRNA）基因序列分析研究，将来自一头患有致命感染的麋鹿（加拿大马鹿，Cervus elaphus canadensis）和一头北美驯鹿（北美林地驯鹿，Rangifer tarandus caribou）的巴贝斯虫分离株，与来自白尾鹿（弗吉尼亚鹿，Odocoileus virginianus）的奥氏巴贝斯虫（Engeling分离株）进行了比较。间接荧光抗体试验和免疫沉淀试验均表明，这些分离株之间存在抗原变异。实验感染研究表明，这些分离株之间没有临床差异。核苷酸序列分析表明，麋鹿和北美驯鹿的巴贝斯虫属分离株所拥有的SSU rRNA基因序列与奥氏巴贝斯虫的序列相同。根据SSU rRNA基因序列构建的系统发育树表明，奥氏巴贝斯虫与分歧巴贝斯虫关系最为密切，二者在真巴贝斯虫进化枝中共同分支。