基于邻菲罗啉荧光猝灭法测定中草药中的亚铁（II）

[Determination of iron(II) in the Chinese herbal medicine based on the fluorescence quenching of phenanthroline].

作者信息

Aodeng G W, Bai W Y, Duan Z Q

机构信息

Institude of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot 010021, China.

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2001 Dec;21(6):846-8.

PMID:12958912

Abstract

A new spectrofluorimetric method for the determination of trace amounts of iron(II) has been developed. This method is based on the fluorescence quenching of phenanthroline due to the formation of complex Fe(II)-phen. In pH = 4.6-7.1 buffer solution, Fe(II) and phen react to form a red complex. Its composition is established by method of continuous variations and molar-ratio as Fe (II): phen = 1:2. The exitation and emission wavelength are found to be 265 nm and 365 nm, respectively. There is a linear relationship in the range of 24-112 ng.mL-1 for Fe(II). The detection limit of Fe(II) is 4.1 ng.mL-1. This method is simple and rapid, and has been applied to the determination of iron in the chinese herbal medicine.

摘要

已开发出一种用于测定痕量铁（II）的新荧光分光光度法。该方法基于由于形成Fe（II）-邻菲罗啉配合物而导致邻菲罗啉的荧光猝灭。在pH = 4.6 - 7.1的缓冲溶液中，Fe（II）与邻菲罗啉反应形成红色配合物。通过连续变化法和摩尔比法确定其组成为Fe（II）：邻菲罗啉 = 1:2。发现激发波长和发射波长分别为265 nm和365 nm。Fe（II）在24 - 112 ng·mL-1范围内呈线性关系。Fe（II）的检测限为4.1 ng·mL-1。该方法简单快速，已应用于中草药中铁的测定。