嗜水气单胞菌几丁质酶对部分N-乙酰化壳聚糖的作用模式

Action pattern of Aeromonas hydrophila chitinase on partially N-acetylated chitosan.

作者信息

Mitsutomi M, Ohtakara A, Fukamizo T, Goto S

机构信息

Department of Applied Biological Sciences, Faculty of Agriculture, Saga University, Japan.

出版信息

Agric Biol Chem. 1990 Apr;54(4):871-7.

PMID:1368549

Abstract

Oligosaccharides from the digestion of 34% N-acetylated chitosan by Aeromonas hydrophila chitinase were separated by CM-Sephadex C-25 column chromatography. Sugar compositions and the sequences of main oligosaccharides were identified through their N-acetylation, their cleavage with exo-glycosidases, and their degradation with nitrous acid. Hetero-chitooligosaccharides such as GlcN.GlcNAc, GlcN.GlcNAc.GlcNAc, GlcNAc.GlcN.GlcNAc, and GlcNAc.GlcN.GlcNAc.GlcNAc, together with GlcNAc and (GlcNAc)2, were detected. The structure of GlcN.GlcNAc was confirmed by the analysis with proton and carbon NMR spectroscopy. These studies indicate that Aeromonas hydrophila chitinase is more specific toward the N-acetyl-beta-D-glucosaminidic bonds in partially N-acetylated chitosan.

摘要

通过CM-葡聚糖凝胶C-25柱色谱法分离嗜水气单胞菌几丁质酶消化34% N-乙酰化壳聚糖产生的寡糖。通过对寡糖进行N-乙酰化、用外切糖苷酶裂解以及用亚硝酸降解，确定了主要寡糖的糖组成和序列。检测到了杂合壳寡糖，如GlcN.GlcNAc、GlcN.GlcNAc.GlcNAc、GlcNAc.GlcN.GlcNAc和GlcNAc.GlcN.GlcNAc.GlcNAc，以及GlcNAc和(GlcNAc)2。通过质子和碳核磁共振光谱分析证实了GlcN.GlcNAc的结构。这些研究表明，嗜水气单胞菌几丁质酶对部分N-乙酰化壳聚糖中的N-乙酰-β-D-葡萄糖胺键具有更高的特异性。