桑（印度桑品种Anantha）叶在链脲佐菌素诱导的糖尿病大鼠中的抗氧化作用。

Antioxidant role of mulberry (Morus indica L. cv. Anantha) leaves in streptozotocin-diabetic rats.

作者信息

Andallu Bondada, Varadacharyulu N Ch

机构信息

Department of Home Science, Sri Sathya Sai Institute of Higher Learning, Anantapur-515001, India.

出版信息

Clin Chim Acta. 2003 Dec;338(1-2):3-10. doi: 10.1016/s0009-8981(03)00322-x.

DOI:10.1016/s0009-8981(03)00322-x

PMID:14637259

Abstract

BACKGROUND

The antihyperglycemic and antioxidant role of mulberry (Morus indica L.) leaves were investigated.

METHODS

Streptozotocin (STZ)-induced diabetic male Wistar rats were used as experimental models; one group was given 25% dry mulberry leaf powder mixed with the standard diet and another group was given standard diet for a period of 8 weeks. The antihyperglycemic and antioxidant role of mulberry was assessed by determining its effect on blood glucose, lipid peroxidation, reduced glutathione (GSH) concentrations and on the activity of glucose-6-phosphate dehydrogenase (G6PDH) and various antioxidant enzymes in erythrocytes and compared with that of controls.

RESULTS

Mulberry-treated diabetic rats showed a significant decrease in fasting blood glucose concentrations indicating a good glycemic control. Increased lipid peroxidation and the activity of catalase (CAT) in erythrocytes observed in diabetic controls were significantly decreased by mulberry leaves (48% and 33%, respectively). Decreased GSH concentrations and the activity of glucose-6-phosphate dehydrogenase and antioxidant enzymes viz., glutathione peroxidase (GPx), glutathione reductase (GR), glutathione-S-transferase (GST) and superoxide dismutase (SOD) observed in uncontrolled diabetes were improved (52%, 69%, 151%, 95%, 24% and 106%) by mulberry treatment very efficiently.

CONCLUSION

Mulberry leaves possess antihyperglycemic and antioxidant properties.

摘要

背景

研究了桑叶（印度桑）的降血糖和抗氧化作用。

方法

将链脲佐菌素（STZ）诱导的糖尿病雄性Wistar大鼠作为实验模型；一组给予含25%干桑叶粉的标准饮食，另一组给予标准饮食，为期8周。通过测定桑叶对血糖、脂质过氧化、还原型谷胱甘肽（GSH）浓度以及红细胞中葡萄糖-6-磷酸脱氢酶（G6PDH）和各种抗氧化酶活性的影响来评估桑叶的降血糖和抗氧化作用，并与对照组进行比较。

结果

用桑叶治疗的糖尿病大鼠空腹血糖浓度显著降低，表明血糖控制良好。糖尿病对照组中观察到的红细胞脂质过氧化增加和过氧化氢酶（CAT）活性，经桑叶处理后显著降低（分别降低48%和33%）。在未得到控制的糖尿病中观察到的GSH浓度降低以及葡萄糖-六磷酸脱氢酶和抗氧化酶即谷胱甘肽过氧化物酶（GPx）、谷胱甘肽还原酶（GR）、谷胱甘肽-S-转移酶（GST）和超氧化物歧化酶（SOD）活性降低，经桑叶治疗后得到非常有效的改善（分别提高52%、69%、151%、95%、24%和106%）。