肝细胞癌的单层动态螺旋CT：影像特征与肿瘤微血管密度的相关性

Single-level dynamic spiral CT of hepatocellular carcinoma: correlation between imaging features and density of tumor microvessels.

作者信息

Chen Wei-Xia, Min Peng-Qiu, Song Bin, Xiao Bong-Liang, Liu Yan, Ge Ying-Hui

机构信息

Department of Radiology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.

出版信息

World J Gastroenterol. 2004 Jan;10(1):67-72. doi: 10.3748/wjg.v10.i1.67.

DOI:10.3748/wjg.v10.i1.67

PMID:14695771

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4717081/

Abstract

AIM

To investigate the correlation of enhancement features of hepatocellular carcinoma (HCC) revealed by single-level dynamic spiral CT scanning (DSCT) with tumor microvessel density (MVD), and to determine the validity of DSCT in assessing in vivo tumor angiogenic activity of HCC.

METHODS

Twenty six HCC patients were diagnosed histopathologically. DSCT was performed for all patients according to standard scanning protocol. Time-density curves were generated, relevant curve parameters were measured, and gross enhancement morphology was analyzed. Operation was performed to remove HCC lesions 1 to 2 weeks following CT scan. Histopathological slides were carefully prepared for the standard F(8)RA immunohistochemical staining and tumor microvessel counting. Enhancement imaging features of HCC lesions were correlatively studied with tumor MVD and its intra-tumor distribution characteristics.

RESULTS

On DSCT images of HCC lesions, three patterns of time-density curve and three types of gross enhancement morphology were recognized. Histomorphologically, the distribution of positively stained tumor endothelial cells within tumor was categorized into 3 types. Curve parameters such as peak enhancement value and contrast enhancement ratio were significantly correlated with tumor tissue MVD (r=0.508 and r=0.423, P<0.01 and P<0.05 respectively). Both the pattern of time-density curve and the gross enhancement morphology of HCC lesions were also correlated with tumor MVD, and reflected the distributive features of tumor microvessels within HCC lesions. Correlation between the likelihood of intrahepatic metastasis of HCC lesions with densely enhanced pseudocapsules and rich pseudocapsular tumor MVD was found.

CONCLUSION

Enhancement imaging features of HCC lesions on DSCT scanning are correlated with tumor MVD, and reflect the intra-tumor distribution characteristics of tumor microvessels. DSCT is valuable in assessing the angiogenic activity and tumor neovascularity of HCC patients in vivo.

摘要

目的

探讨单层动态螺旋CT扫描（DSCT）显示的肝细胞癌（HCC）增强特征与肿瘤微血管密度（MVD）的相关性，并确定DSCT在评估HCC体内肿瘤血管生成活性方面的有效性。

方法

26例HCC患者经组织病理学确诊。所有患者均按照标准扫描方案进行DSCT检查。生成时间-密度曲线，测量相关曲线参数，并分析总体增强形态。在CT扫描后1至2周进行手术切除HCC病灶。精心制备组织病理切片用于标准F(8)RA免疫组织化学染色和肿瘤微血管计数。对HCC病灶的增强成像特征与肿瘤MVD及其肿瘤内分布特征进行相关性研究。

结果

在HCC病灶DSCT图像上，识别出三种时间-密度曲线模式和三种总体增强形态类型。组织形态学上，肿瘤内阳性染色的肿瘤内皮细胞分布分为3种类型。峰值增强值和对比增强率等曲线参数与肿瘤组织MVD显著相关（r分别为0.508和0.423，P均<0.01和P<0.05）。HCC病灶的时间-密度曲线模式和总体增强形态也与肿瘤MVD相关，并反映了HCC病灶内肿瘤微血管的分布特征。发现HCC病灶肝内转移可能性与强化浓密的假包膜及丰富的假包膜肿瘤MVD之间存在相关性。