猪带绦虫遗传变异的检测
Detection of genetic variation in Taenia solium.
作者信息
Maravilla Pablo, Souza Valeria, Valera Aldo, Romero-Valdovinos Mirza, Lopez-Vidal Yolanda, Dominguez-Alpizar Jose Luis, Ambrosio Javier, Kawa Simon, Flisser Ana
机构信息
Direccion de Investigacion, Hospital General Dr. Manuel Gea Gonzalez, SSA, 11400 Mexico, DF, Mexico.
出版信息
J Parasitol. 2003 Dec;89(6):1250-4. doi: 10.1645/GE-2786RN.
Genetic variability among Taenia solium isolates was studied in 160 cysticerci from 6 pigs, 4 from Mexico, 1 from Honduras, and 1 from Tanzania. Random amplified polymorphic DNA (RAPD) analysis performed with 4 commercial primers showed 88% polymorphic loci and an average heterozygosity of 0.077; however, several alleles were fixed within each isolate. Linkage disequilibrium analysis indicated that 3 of the 6 isolates had a random association of alleles, whereas the other 3 had a clonal structure. These results suggest the existence of local lineages in T. solium, with events of genetic recombination within them.
对来自6头猪的160个猪带绦虫囊尾蚴进行了研究，这些猪分别来自墨西哥（4头）、洪都拉斯（1头）和坦桑尼亚（1头），以分析猪带绦虫分离株之间的遗传变异性。使用4种商业引物进行的随机扩增多态性DNA（RAPD）分析显示，多态性位点为88%，平均杂合度为0.077；然而，每个分离株内有几个等位基因是固定的。连锁不平衡分析表明，6个分离株中有3个等位基因呈随机关联，而其他3个具有克隆结构。这些结果表明猪带绦虫中存在本地谱系，并且在谱系内部存在基因重组事件。
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