采用高效液相色谱-质谱联用（HPLC-MS）和气相色谱-质谱联用（GC-MS）对圣约翰草（贯叶连翘）超临界流体提取物进行表征分析。

Characterization of supercritical fluid extracts of St. John's wort (Hypericum perforatum L.) by HPLC-MS and GC-MS.

作者信息

Seger Christoph, Römpp Harald, Sturm Sonja, Haslinger Ernst, Schmidt Peter C, Hadacek Franz

机构信息

Department of Pharmaceutical Technology, Institute of Pharmacy, Leopold-Franzens University, Innsbruck, Austria.

出版信息

Eur J Pharm Sci. 2004 Mar;21(4):453-63. doi: 10.1016/j.ejps.2003.10.021.

DOI:10.1016/j.ejps.2003.10.021

PMID:14998575

Abstract

Supercritical fluid extracts (carbon dioxide without modifiers) of St. John's Wort (Hypericum perforatum L., Clusiaceae) were analyzed by GC-MS, HPLC-DAD and HPLC-DAD-MS. Besides the dominating phloroglucinols hyperforin (36.5 +/- 1.1%) and adhyperforin (4.6 +/- 0.1%), the extracts mainly contained alkanes (predominantly nonacosane), fatty acids and wax esters. The apolar components tended to accumulate in a waxy phase resting a top of the hyperforin-enriched phase. No components of higher polarity like naphthodianthrones were found. A set of hyperforin oxidation products was detected and tentatively assigned using HPLC-MS.

摘要

采用气相色谱 - 质谱联用（GC-MS）、高效液相色谱 - 二极管阵列检测（HPLC-DAD）和高效液相色谱 - 二极管阵列 - 质谱联用（HPLC-DAD-MS）对贯叶连翘（金丝桃科贯叶连翘属植物贯叶连翘L.）的超临界流体提取物（无改性剂的二氧化碳提取物）进行了分析。除了占主导地位的间苯三酚类化合物金丝桃素（36.5±1.1%）和去甲金丝桃素（4.6±0.1%）外，提取物主要含有烷烃（主要是二十九烷）、脂肪酸和蜡酯。非极性成分倾向于在富含金丝桃素的相顶部的蜡相中积累。未发现更高极性的成分，如萘骈二蒽酮。利用HPLC-MS检测并初步鉴定了一组金丝桃素氧化产物。