模仿枝原体16S - 23S rRNA基因间隔区中的一个假定转座酶基因。
A putative transposase gene in the 16S-23S rRNA intergenic spacer region of Mycoplasma imitans.
作者信息
Harasawa Ryô, Pitcher David G, Ramírez Ana S, Bradbury Janet M
机构信息
Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan.
Respiratory and Systemic Infection Laboratory, Health Protection Agency, 61 Colindale Avenue, London NW9 5HT, UK.
出版信息
Microbiology (Reading). 2004 Apr;150(Pt 4):1023-1029. doi: 10.1099/mic.0.26629-0.
Examination of the nucleotide sequences of the 16S-23S intergenic transcribed spacer (ITS) region of Mycoplasma imitans and Mycoplasma gallisepticum identified a putative transposase gene located only in the ITS of M. imitans, which can be used as a genetic marker to distinguish these two species. The relative size of the PCR products of the ITS region allowed a clear distinction to be made between strains of M. imitans and M. gallisepticum, both of which could be readily discriminated from the type strains of all the other recognized avian Mycoplasma species. In addition, the putative transposase gene assigned in the ITS of M. imitans was shown to include a sequence homologous to that of the P75 gene of M. gallisepticum. This is believed to be the first description of an insertion element in the rRNA operon region of a mycoplasma species.
对模仿支原体和鸡毒支原体16S - 23S基因间隔转录间隔区（ITS）的核苷酸序列进行检测后发现，一个假定的转座酶基因仅存在于模仿支原体的ITS中，该基因可作为区分这两个物种的遗传标记。ITS区域PCR产物的相对大小使得模仿支原体菌株和鸡毒支原体菌株之间能够清晰区分，这两种菌株也都能轻易地与所有其他已确认的禽支原体种类的模式菌株区分开来。此外，在模仿支原体ITS中发现的假定转座酶基因显示包含一段与鸡毒支原体P75基因序列同源的序列。据信，这是首次对支原体物种rRNA操纵子区域中的插入元件进行描述。
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