异核编辑NOESY光谱中对角峰抑制的通用方法。

General method for suppression of diagonal peaks in heteronuclear-edited NOESY spectroscopy.

作者信息

Wu Jihui, Fan Jing-song, Pascal Steven M, Yang Daiwen

机构信息

Department of Biological Sciences and Department of Chemistry, National University of Singapore, 14 Science Drive 4, Singapore 117543.

出版信息

J Am Chem Soc. 2004 Nov 24;126(46):15018-9. doi: 10.1021/ja045300l.

DOI:10.1021/ja045300l

PMID:15547985

Abstract

A novel approach is proposed for suppression of diagonal peaks in 15N- and 13C-edited NOESY spectroscopy based on subtracting a spectrum with only diagonal peaks from the conventional NOESY spectrum. This method can be applied to most heteronuclear-edited NOESY experiments. It is far more sensitive than the TROSY-based approach for biomolecules with little TROSY effect, and nearly complete suppression of diagonal peaks can be achieved. The method has been demonstrated on samples of 15N-labeled calmodulin (17 kDa), 13C-labeled DdCAD-1 (24 kDa), and 13C-labeled hemoglobin (65 kDa), showing that cross-peaks very close to diagonals can be assigned reliably based on the difference spectra.

摘要

提出了一种基于从传统NOESY谱中减去仅含对角峰的谱来抑制15N和13C编辑的NOESY谱中对角峰的新方法。该方法可应用于大多数异核编辑的NOESY实验。对于几乎没有TROSY效应的生物分子，它比基于TROSY的方法灵敏得多，并且可以实现对角峰的几乎完全抑制。该方法已在15N标记的钙调蛋白（17 kDa）、13C标记的DdCAD-1（24 kDa）和13C标记的血红蛋白（65 kDa）样品上得到验证，表明基于差异谱可以可靠地归属非常靠近对角线的交叉峰。