通过流式荧光原位杂交技术测定唐氏综合征患者的端粒长度。

Determination of telomere length by flow-fluorescence in situ hybridization in Down's syndrome patients.

作者信息

Brando B, Longo A, Beltrami B, Passoni D, Verna R, Licastro F, Corsi M M

机构信息

Laboratory of Transplant Immunology and Hematologic Diagnosis, Hospital Niguarda Ca' Granda, Milan, Italy.

出版信息

Int J Tissue React. 2004;26(1-2):61-4.

PMID:15573694

Abstract

A new method for measuring telomere length in a population of Down's syndrome patients aged 18-60 years old is presented. The method is based on flow cytometry and quantitative fluorescence in situ hybridization (flow-FISH) on whole cells. At least three methods for measuring the length of telomere repeats have been described: (i) Southern blot analysis, and quantitative FISH using either (ii) digital fluorescence microscopy (Q-FISH) or (iii) flow cytometry (flow-FISH). Both Southern blot analysis and Q-FISH have specific limitations and are time-consuming, whereas flow-FISH needed relatively few cells (1.5-2.5 x 106) and could be completed in 24-48 h. The method can be used to rapidly determine telomere length in subsets of nucleated blood cells from patients with age-related diseases such as Down's syndrome, Alzheimer's disease and Werner syndrome.

摘要

本文介绍了一种测量18至60岁唐氏综合征患者群体中端粒长度的新方法。该方法基于全细胞的流式细胞术和定量荧光原位杂交（flow-FISH）。目前已描述了至少三种测量端粒重复序列长度的方法：（i）Southern印迹分析，以及使用（ii）数字荧光显微镜（Q-FISH）或（iii）流式细胞术（flow-FISH）的定量FISH。Southern印迹分析和Q-FISH都有特定的局限性且耗时，而flow-FISH所需细胞相对较少（1.5 - 2.5×10⁶），且可在24至48小时内完成。该方法可用于快速确定患有唐氏综合征、阿尔茨海默病和沃纳综合征等与年龄相关疾病患者有核血细胞亚群中的端粒长度。