奶牛围产期血液和乳汁中多形核白细胞上黏附分子和归巢受体的表达

Adhesion molecule and homing receptor expression on blood and milk polymorphonuclear leukocytes during the periparturient period of dairy cattle.

作者信息

Harp James A, Waters Theresa E, Goff Jesse P

机构信息

Periparturient Diseases of Cattle Research Unit, USDA-ARS, National Animal Disease Center, Ames, IA 50010, USA.

出版信息

Vet Immunol Immunopathol. 2005 Mar 10;104(1-2):99-103. doi: 10.1016/j.vetimm.2004.10.007.

DOI:10.1016/j.vetimm.2004.10.007

PMID:15661335

Abstract

Adhesion molecule and homing receptor expression on blood and milk polymorphonuclear leukocytes (PMN) from periparturient dairy cattle was studied. Both percentages and the mean fluorescence intensity (MFI) of PMN expressing CD11a, CD44, CD62L, and LPAM-1 (alpha4 beta7) were evaluated at seven time points during the twenty-one day period post calving. CD11a and CD62L were expressed on 94-100% of PMN in both blood and milk and there were no significant differences in these percentages at any time point. LPAM-1 was expressed on 3-10% of the PMN in the blood and 13-45% in the milk and the percentage of cells expressing LPAM-1 in milk was significantly (P<0.05) greater than in blood at 0, 4, 10, 14, 18 and 21 days after calving. CD44 was expressed on 11-39% of the PMN in blood and 33-69% in the milk and the percentage of cells expressing CD44 in milk was significantly (P<0.05) greater than in blood at all time points. The MFI of CD11a on milk PMN was consistently higher than that of blood PMN throughout the study period and significantly (P<0.05) higher at days 4, 10 and 18 after calving.

摘要

研究了围产期奶牛血液和乳汁中多形核白细胞（PMN）上黏附分子和归巢受体的表达。在产犊后21天的7个时间点评估了表达CD11a、CD44、CD62L和LPAM-1（α4β7）的PMN的百分比和平均荧光强度（MFI）。血液和乳汁中94%-100%的PMN表达CD11a和CD62L，在任何时间点这些百分比均无显著差异。血液中3%-10%的PMN表达LPAM-1，乳汁中为13%-45%，产犊后0、4、10、14、18和21天，乳汁中表达LPAM-1的细胞百分比显著高于血液（P<0.05）。血液中11%-39%的PMN表达CD44，乳汁中为33%-69%，在所有时间点，乳汁中表达CD44的细胞百分比均显著高于血液（P<0.05）。在整个研究期间，乳汁PMN上CD11a的MFI始终高于血液PMN，产犊后第4、10和18天显著更高（P<0.05）。