Singer Andrew C, van der Gast Christopher J, Thompson Ian P
Environmental Biotechnology Section, Centre for Ecology and Hydrology - Oxford, Oxford OX1 3SR, UK.
Trends Biotechnol. 2005 Feb;23(2):74-7. doi: 10.1016/j.tibtech.2004.12.012.
Notwithstanding the phenomenally large and ever-increasing resource of pollutant-degrading microbial isolates in laboratories around the globe, inoculum survival remains the 'Achilles' heel' for bioaugmentation of contaminated land. Considerable effort has been invested into inoculum strain selection to facilitate pollutant biodegradation, ranging from the isolation of 'superbugs,' which are microorganisms highly resilient to environmental stresses, harboring catabolically superior pollutant-degrading enzymes, to the other extreme in 'priming', where pollutant degradation is carried out through the addition of soil enriched with an undefined consortium of pollutant-degrading microorganisms.
尽管全球实验室中用于降解污染物的微生物分离株资源极其丰富且不断增加,但接种体的存活仍然是污染土地生物强化的“阿喀琉斯之踵”。为促进污染物的生物降解,人们在接种菌株的选择上投入了大量精力:从分离“超级细菌”,即对环境压力具有高度抗性、携带降解污染物能力更强的分解代谢酶的微生物,到另一个极端的“引发”,即通过添加富含未定义的降解污染物微生物群落的土壤来进行污染物降解。