使用限制性内切酶EcoRI对幽门螺杆菌进行脉冲场凝胶电泳分析。
Use of the restriction enzyme EcoRI for pulsed-field gel electrophoretic analysis of Helicobacter pylori.
作者信息
Hosaka Yoshio, Irinoda Kazuhiko, Nakano Ryuichi, Tanabe Satosi, Koizumi Wasaburo, Saigenji Katunori, Inoue Matsuhisa
机构信息
Department of Microbiology, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara-shi, Kanagawa 228, Japan.
出版信息
J Clin Microbiol. 2005 Feb;43(2):931-2. doi: 10.1128/JCM.43.2.931-932.2005.
Abstract
Pulsed-field gel electrophoretic (PFGE) analysis of Helicobacter pylori isolates is not commonly employed because of the inability to compare the typing with other typing systems. We adapted the PFGE analysis for H. pylori by using EcoRI and slightly modified our laboratory methods to improve the typing of isolates (typeability was 97%).
摘要
由于无法将幽门螺杆菌分离株的脉冲场凝胶电泳（PFGE）分型结果与其他分型系统进行比较，因此该分析方法并不常用。我们通过使用EcoRI对幽门螺杆菌的PFGE分析方法进行了改进，并对实验室方法进行了微调，以提高分离株的分型能力（分型成功率为97%）。
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