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枯草芽孢杆菌同时选择性生产果聚糖和聚γ-谷氨酸。

Simultaneous and selective production of levan and poly(gamma-glutamic acid) by Bacillus subtilis.

作者信息

Shih Ing-Lung, Yu Yun-Ti

机构信息

Department of Environmental Engineering, Da-Yeh University, Chang-Hwa, Taiwan.

出版信息

Biotechnol Lett. 2005 Jan;27(2):103-6. doi: 10.1007/s10529-004-6936-z.

Abstract

Bacillus subtilis(natto) Takahashi, used to prepare the fermented soybean product natto, was grown in a basal medium containing 5% (w/w) sucrose and 1.5% (w/w) L-glutamate and produced 58% (w/w) poly(gamma-glutamic acid) and 42% (w/w) levan simultaneously. After 21 h, 40-50 mg levan ml-1 had been produced in medium containing 20% (w/w) sucrose but without L-glutamate. In medium containing L-glutamic acid but without sucrose, mainly poly(gamma-glutamic acid) was produced.

摘要

用于制备发酵豆制品纳豆的枯草芽孢杆菌(纳豆)高桥菌株,在含有5%(w/w)蔗糖和1.5%(w/w)L-谷氨酸的基础培养基中生长,同时产生了58%(w/w)的聚(γ-谷氨酸)和42%(w/w)的果聚糖。21小时后,在含有20%(w/w)蔗糖但不含L-谷氨酸的培养基中产生了40 - 50毫克果聚糖/毫升。在含有L-谷氨酸但不含蔗糖的培养基中,主要产生聚(γ-谷氨酸)。

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