雌激素、他莫昔芬和表皮生长因子对乳腺癌细胞中血管内皮生长因子转录调控的影响。
Effect of estrogen, tamoxifen and epidermal growth factor on the transcriptional regulation of vascular endothelial growth factor in breast cancer cells.
作者信息
Lee Jeong Eon, Chung Ki-Wook, Han Wonshik, Kim Sung-Won, Kim Seok Won, Shin Hyuk Jai, Bae Ji Yeoun, Noh Dong-Young
机构信息
Department of Surgery, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul 110-744, Korea.
出版信息
Anticancer Res. 2004 Nov-Dec;24(6):3961-4.
BACKGROUND
VEGF (Vascular Endothelial Growth Factor) is a key factor of angiogenesis and high tissue VEGF levels are related to a poor prognosis in breast cancer.
MATERIALS AND METHODS
By semi-quantitative RT-PCR, we determined the relative expressions of VEGF mRNA in MCF-7 (both ER-alpha+ and ER-beta+ (mainly ER-alpha+), PR+, bcl-2+, EGFR-) and MB-MDA-231 (only ER-beta+, PR-, EGFR-) breast cancer cells which were treated with estrogen, tamoxifen and EGF (Epidermal Growth Factor).
RESULTS
In MCF-7 cell lines, estrogen induced the expression of VEGF mRNA while tamoxifen reduced its expression. Estrogen and tamoxifen did not confer any significant effect on MB-MDA-231 cells and EGF showed no significant effect on MCF-7 or MB-MDA-231.
CONCLUSION
Reduced VEGF mRNA expression of MCF-7 cells treated with tamoxifen may be related to the antagonistic effect of tamoxifen on ER-positive breast cancer, and this antagonistic effect may be related to ER-alpha.
背景
血管内皮生长因子（VEGF）是血管生成的关键因子，组织中VEGF水平升高与乳腺癌预后不良相关。
材料与方法
通过半定量逆转录聚合酶链反应（RT-PCR），我们测定了雌激素、他莫昔芬和表皮生长因子（EGF）处理后的MCF-7（雌激素受体α阳性和雌激素受体β阳性（主要为雌激素受体α阳性）、孕激素受体阳性、bcl-2阳性、表皮生长因子受体阴性）和MB-MDA-231（仅雌激素受体β阳性、孕激素受体阴性、表皮生长因子受体阳性）乳腺癌细胞中VEGF mRNA的相对表达。
结果
在MCF-7细胞系中，雌激素诱导VEGF mRNA表达，而他莫昔芬降低其表达。雌激素和他莫昔芬对MB-MDA-231细胞无显著影响，EGF对MCF-7或MB-MDA-23没有显著影响。
结论
他莫昔芬处理的MCF-7细胞中VEGF mRNA表达降低可能与他莫昔芬对雌激素受体阳性乳腺癌的拮抗作用有关，且这种拮抗作用可能与雌激素受体α有关。
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