结缔组织生长因子在博来霉素诱导的小鼠肺纤维化中的初步研究
[A preliminary study of CTGF in bleomycin-induced mouse pulmonary fibrosis].
作者信息
Zhang Lei, Lu Hui-ping, Lai Guo-xiang
机构信息
Department of Respiratory Disease, Fuzhou General Hospital of Nanjing Military Command, Fuzhou 350025, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2005 May;21(3):290-2.
AIM
To investigate CTGF's role in bleomycin-induced pulmonary fibrosis.
METHODS
Kunming mice were intratracheally administered with bleomycin to establish pulmonary fibrosis model. The expression of CTGF was studied by immunohistochemical method and RT-PCR. The degree of fibrosis was evaluated by pulmonary hydroxyproline assay.
RESULTS
CTGF protein and mRNA were not expressed in normal mice. However, they were detected in pulmonary fibrotic mice from day 7 after bleomycin treatment, and increased as fibrosis developed (P <0.01). CTGF protein's expression was correlated with hydroxyproline content in lung tissue (r=0.92, P <0.01).
CONCLUSION
CTGF plays a role in pulmonary fibrosis. Detection of CTGF may be an early and sensitive marker for evaluating the occurrence and development of pulmonary fibrosis.
目的
探讨结缔组织生长因子（CTGF）在博来霉素诱导的肺纤维化中的作用。
方法
将博来霉素经气管内注入昆明小鼠以建立肺纤维化模型。采用免疫组织化学法和逆转录-聚合酶链反应（RT-PCR）研究CTGF的表达。通过肺羟脯氨酸测定评估纤维化程度。
结果
正常小鼠未检测到CTGF蛋白和mRNA。然而，在博来霉素治疗后第7天起在肺纤维化小鼠中检测到它们，且随着纤维化发展而增加（P<0.01）。CTGF蛋白的表达与肺组织中羟脯氨酸含量相关（r=0.92，P<0.01）。
结论
CTGF在肺纤维化中起作用。检测CTGF可能是评估肺纤维化发生和发展的早期敏感标志物。
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