通过监测严重联合免疫缺陷（SCID）小鼠的卵囊排出量来定量微小隐孢子虫的感染性。

Quantification of the infectivity of Cryptosporidium parvum by monitoring the oocyst discharge from SCID mice.

作者信息

Hikosaka Kenji, Satoh Masaaki, Koyama Yukari, Nakai Yutaka

机构信息

Laboratory of Animal Health and Management, Graduate School of Agricultural Science, Tohoku University, Naruko-cho, Miyagi 989-6711, Japan.

出版信息

Vet Parasitol. 2005 Nov 25;134(1-2):173-6. doi: 10.1016/j.vetpar.2005.06.017. Epub 2005 Aug 1.

DOI:10.1016/j.vetpar.2005.06.017

PMID:16076530

Abstract

Doses of 1-10(5) oocysts of Cryptosporidium parvum HNJ-1 were inoculated into severe combined immunodeficient (SCID) mice, and the discharge of oocysts was monitored for 30 days post inoculation. None of the mice discharged any oocysts after oral inoculation of one oocyst. Only one of five SCID mice discharged oocysts after oral inoculation of 10 oocysts, and the prepatent period was 17 days. The other four mice did not discharge any oocysts. All the SCID mice discharged oocysts after oral inoculation of 10(2)-10(5) oocysts. The prepatent periods were 13-17, 8-10, 8, and 10 days in SCID mice inoculated with 10(2), 10(3), 10(4), and 10(5) oocysts, respectively. A proportional correlation was observed between inoculation doses of oocysts ranging from 10 to 10(4) oocysts and the corresponding prepatent periods. The prepatent period can be used to evaluate the infectivity of C. parvum oocysts.

摘要

将1 - 10⁵个微小隐孢子虫HNJ - 1卵囊接种到严重联合免疫缺陷（SCID）小鼠体内，并在接种后30天监测卵囊排出情况。口服接种1个卵囊后，没有小鼠排出任何卵囊。口服接种10个卵囊后，5只SCID小鼠中只有1只排出卵囊，潜伏期为17天。其他4只小鼠未排出任何卵囊。口服接种10² - 10⁵个卵囊后，所有SCID小鼠均排出卵囊。接种10²、10³、10⁴和10⁵个卵囊的SCID小鼠的潜伏期分别为13 - 17天、8 - 10天、8天和10天。在接种剂量为10至10⁴个卵囊的范围内，观察到卵囊接种剂量与相应潜伏期之间存在比例相关性。潜伏期可用于评估微小隐孢子虫卵囊的感染性。