重水（D2O）对人胰腺肿瘤细胞的影响。
Effects of heavy water (D2O) on human pancreatic tumor cells.
作者信息
Hartmann Johannes, Bader Yvonne, Horvath Zsuzsanna, Saiko Philipp, Grusch Michael, Illmer Christoph, Madlener Sibylle, Fritzer-Szekeres Monika, Heller Nicole, Alken Rudolf-Giesbert, Szekeres Thomas
机构信息
Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Vienna, General Hospital of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria.
出版信息
Anticancer Res. 2005 Sep-Oct;25(5):3407-11.
BACKGROUND
Pancreatic cancer constitutes an entity which is difficult to treat and, therefore, mostly fatal. Since heavy water (deuterium oxide, D2O) was shown to be active in various cancer cell lines in vitro and in vivo, we now investigated its effects in human pancreatic tumor cells.
MATERIALS AND METHODS
The cytotoxic effects of D2O were examined in three pancreatic cancer cell lines (AsPC-1, BxPC-3 and PANC-1). Induction of apoptosis was determined by Hoechst/propidium iodide double staining and cell cycle distribution was investigated by FACS analysis.
RESULTS
Employing a clonogenic assay, D2O yielded IC50 values of 15%, 18% and 27% in AsPC-1, PANC-1 and BxPC-3 cells, respectively, and led to the induction of apoptosis when compared to untreated controls. Moreover, D2O caused a cell cycle arrest in the G2-M-phase (BxPC-3, PANC-1) or in the S-phase (AsPC-1).
CONCLUSION
It is hoped that D2O might offer an additional option for the treatment of pancreatic carcinomas.
背景
胰腺癌是一种难以治疗的疾病，因此大多是致命的。由于重水（氧化氘，D2O）在体外和体内的各种癌细胞系中均显示出活性，我们现在研究了其对人胰腺肿瘤细胞的影响。
材料与方法
检测了D2O对三种胰腺癌细胞系（AsPC-1、BxPC-3和PANC-1）的细胞毒性作用。通过Hoechst/碘化丙啶双重染色确定凋亡诱导情况，并通过流式细胞术分析研究细胞周期分布。
结果
采用克隆形成试验，D2O在AsPC-1、PANC-1和BxPC-3细胞中的IC50值分别为15%、18%和27%，与未处理的对照相比，可诱导细胞凋亡。此外，D2O导致细胞周期阻滞在G2-M期（BxPC-3、PANC-1）或S期（AsPC-1）。
结论
希望D2O可能为胰腺癌的治疗提供一种额外的选择。
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