质粒编码的A类碳青霉烯水解β-内酰胺酶KPC-3的动力学研究
Kinetics study of KPC-3, a plasmid-encoded class A carbapenem-hydrolyzing beta-lactamase.
作者信息
Alba Jimena, Ishii Yoshikazu, Thomson Kenneth, Moland Ellen Smith, Yamaguchi Keizo
机构信息
Department of Microbiology, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku, Tokyo 1438540, Japan.
出版信息
Antimicrob Agents Chemother. 2005 Nov;49(11):4760-2. doi: 10.1128/AAC.49.11.4760-4762.2005.
Abstract
The kinetic activity of KPC-3, a plasmid-encoded class A carbapenemase, was studied. It hydrolyzed penicillins, cephalosporins, carbapenems, and even sulbactam. The best substrate was cephalothin (k(cat/K)m = 3.48 microM(-1) s(-1)). The efficiency of the enzyme was similar for imipenem and meropenem (k(cat)/K(m), 1.4 and 1.94 microM(-1) s(-1), respectively).
摘要
对质粒编码的A类碳青霉烯酶KPC-3的动力学活性进行了研究。它能水解青霉素、头孢菌素、碳青霉烯类，甚至舒巴坦。最佳底物是头孢噻吩（k(cat/K)m = 3.48 microM(-1) s(-1)）。该酶对亚胺培南和美罗培南的催化效率相似（k(cat)/K(m)分别为1.4和1.94 microM(-1) s(-1)）。
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