膜片荧光测定法：为离子通道研究带来新曙光。
Patch fluorometry: shedding new light on ion channels.
作者信息
Zheng Jie
机构信息
Department of Physiology and Membrane Biology, University of California School of Medicine, Davis, California, USA.
出版信息
Physiology (Bethesda). 2006 Feb;21:6-12. doi: 10.1152/physiol.00041.2005.
PMID:16443817
Abstract
Patch fluorometry has emerged as a new approach to the study of the structure-function relationship in membrane-embedded functional ion channels. Simultaneous fluorescent and electrical recordings are achieved from a small number of channels in a cell-free membrane patch, yielding high recording sensitivities. Further improvement of this approach should permit direct observation of the gating motion of a single-channel protein.
摘要
膜片荧光测定法已成为研究膜嵌入功能性离子通道结构-功能关系的一种新方法。通过在无细胞的膜片中对少量通道进行荧光和电信号同步记录，可获得高记录灵敏度。该方法的进一步改进应能直接观察单通道蛋白的门控运动。
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