嗜热栖热菌翻译起始因子1刺激翻译起始复合物的形成。
Sulfolobus solfataricus translation initiation factor 1 stimulates translation initiation complex formation.
作者信息
Hasenöhrl David, Benelli Dario, Barbazza Alessandra, Londei Paola, Bläsi Udo
机构信息
Max F. Perutz Laboratories, Department of Microbiology and Immunobiology, University Departments at the Vienna Biocenter, Dr. Bohrgasse 9/4, 1030 Vienna, Austria.
出版信息
RNA. 2006 Apr;12(4):674-82. doi: 10.1261/rna.2289306. Epub 2006 Mar 3.
Abstract
The eukaryotic translation initiation factor 1 binds to the ribosome during translation initiation. It is instrumental for initiator-tRNA and mRNA binding, and has a function in selection of the authentic start codon. Here, we show that the archaeal homolog aIF1 has analogous functions. The aIF1 protein of the archaeon Sulfolobus solfataricus is bound to the small ribosomal subunit during translation initiation and accelerates binding of initiator-tRNA and mRNA to the ribosome. Accordingly, aIF1 stimulated translation of an mRNA in a S. solfataricus in vitro translation system. Moreover, this study suggested that the C terminus of the factor is of relevance for its function.
摘要
真核生物翻译起始因子1在翻译起始过程中与核糖体结合。它对起始tRNA和mRNA的结合起重要作用，并在选择正确的起始密码子中发挥功能。在此，我们表明古细菌同源物aIF1具有类似功能。嗜热栖热菌的aIF1蛋白在翻译起始过程中与小核糖体亚基结合，并加速起始tRNA和mRNA与核糖体的结合。因此，aIF1在嗜热栖热菌体外翻译系统中刺激了mRNA的翻译。此外，这项研究表明该因子的C末端与其功能相关。
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