吴茱萸次碱通过抑制RAW 264.7巨噬细胞中NF-κB的激活来抑制脂多糖诱导的诱导型一氧化氮合酶和环氧化酶-2的表达。
Inhibition of lipopolysaccharide-induced iNOS and COX-2 expression by dehydroevodiamine through suppression of NF-kappaB activation in RAW 264.7 macrophages.
作者信息
Noh Eun Jung, Ahn Kwang Seok, Shin Eun Myoung, Jung Sang Hoon, Kim Yeong Shik
机构信息
Natural Products Research Institute, College of Pharmacy, Seoul National University, 28 Yeongun-dong, Jongno-Ku, Seoul 110-460, South Korea.
出版信息
Life Sci. 2006 Jul 10;79(7):695-701. doi: 10.1016/j.lfs.2006.02.020. Epub 2006 Mar 6.
Dehydroevodiamine is a major bioactive quinazoline alkaloid isolated from Evodiae Fructus. We investigated the anti-inflammatory properties of dehydroevodiamine in RAW 264.7 murine macrophages. The results indicated that dehydroevodiamine inhibited the expression of LPS-induced iNOS and COX-2 proteins and suppressed also their mRNAs from RT-PCR experiment on RAW 264.7 cells. Furthermore, this compound inhibited the level of LPS-stimulated prostaglandin E2 (PGE2) and LPS-induced nuclear factor-kappa B (NF-kappaB). Therefore, we suggested that the effect of dehydroevodiamine-mediated inhibition of the expression LPS-induced iNOS and COX-2 genes is due to under the suppression of NF-kappaB activation in the transcriptional level.
吴茱萸碱是从吴茱萸果实中分离出的一种主要生物活性喹唑啉生物碱。我们研究了吴茱萸碱在RAW 264.7小鼠巨噬细胞中的抗炎特性。结果表明，吴茱萸碱抑制了脂多糖（LPS）诱导的诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）蛋白的表达，并且在对RAW 264.7细胞进行的逆转录聚合酶链反应（RT-PCR）实验中也抑制了它们的信使核糖核酸（mRNA）。此外，该化合物抑制了LPS刺激的前列腺素E2（PGE2）水平以及LPS诱导的核因子-κB（NF-κB）。因此，我们认为吴茱萸碱介导的对LPS诱导的iNOS和COX-2基因表达的抑制作用是由于在转录水平上抑制了NF-κB的激活。
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