来自 Aphrodisiaca 异翅藤的一种脂肪族硝基化合物的体外抗病毒活性。
The in vitro antiviral activity of an aliphatic nitro compound from Heteropteris aphrodisiaca.
作者信息
Melo Fernando L, Benati Fabricio J, Roman Walter Antonio, de Mello João Carlos Palazzo, Nozawa Carlos, Linhares Rosa Elisa Carvalho
机构信息
Departamento de Microbiologia/CCB, Universidade Estadual de Londrina, Caixa Postal 6001, CEP 86051-990 Londrina-Paraná, Brazil.
出版信息
Microbiol Res. 2008;163(2):136-9. doi: 10.1016/j.micres.2006.03.011. Epub 2006 Jun 2.
We investigated the antiviral activity of an aliphatic nitro compound (NC) isolated from Heteropteris aphrodisiaca O. Mach. (Malpighiaceae), a Brazilian medicinal plant. The NC was tested for its antiviral activity against poliovirus type 1 (PV-1) and bovine herpes virus type 1 (BHV-1) by plaque reduction assay in cell culture. The NC showed a moderate antiviral activity against PV-1 and BHV-1 in HEp-2 cells, and the 50% inhibitory concentration (IC50) were 22.01 microg/ml (selectivity index (SI)=2.83) and 21.10 microg/ml (SI=2.95), respectively. At the highest concentration of the drug (40 microg/ml) a reduction of approximately 80% in plaque assay was observed for both viruses. The treatment of cells or virus prior to infection did not inhibit the replication of virus strains.
我们研究了从巴西药用植物 Heteropteris aphrodisiaca O. Mach.（金虎尾科）中分离出的一种脂肪族硝基化合物（NC）的抗病毒活性。通过细胞培养中的蚀斑减少试验，检测了该 NC 对 1 型脊髓灰质炎病毒（PV-1）和 1 型牛疱疹病毒（BHV-1）的抗病毒活性。在 HEp-2 细胞中，NC 对 PV-1 和 BHV-1 表现出中等抗病毒活性，50%抑制浓度（IC50）分别为 22.01 微克/毫升（选择性指数（SI）=2.83）和 21.10 微克/毫升（SI=2.95）。在药物最高浓度（40 微克/毫升）下，两种病毒在蚀斑试验中均观察到约 80%的减少。感染前对细胞或病毒进行处理并未抑制病毒株的复制。
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