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携带志贺毒素编码基因的噬菌体在反刍动物体内的转导

In vivo transduction of an Stx-encoding phage in ruminants.

作者信息

Cornick Nancy A, Helgerson Amy F, Mai Volker, Ritchie Jennifer M, Acheson David W K

机构信息

Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA 50010, USA.

出版信息

Appl Environ Microbiol. 2006 Jul;72(7):5086-8. doi: 10.1128/AEM.00157-06.

Abstract

We assessed the ability of a kanamycin-marked Stx phage to move into a commensal, ovine Escherichia coli strain in the ruminant gastrointestinal tract. Transduction was detected in 19/24 sheep tested, resulting in the recovery of 47 transductants. Subtherapeutic doses of the quinolone antibiotic enrofloxacin did not increase the rate of transduction.

摘要

我们评估了一种卡那霉素标记的志贺毒素噬菌体在反刍动物胃肠道中转移至共生绵羊大肠杆菌菌株的能力。在24只受试绵羊中的19只检测到了转导,共获得47个转导子。喹诺酮类抗生素恩诺沙星的亚治疗剂量并未提高转导率。

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