制备多功能糖纳米颗粒作为潜在的基于碳水化合物的抗癌疫苗平台。

Preparation of multifunctional glyconanoparticles as a platform for potential carbohydrate-based anticancer vaccines.

作者信息

Ojeda Rafael, de Paz Jose Luis, Barrientos Africa G, Martín-Lomas Manuel, Penadés Soledad

机构信息

Grupo de Carbohidratos, Laboratory of Glyconanotechnology, Instituto de Investigaciones Químicas-CSIC, Américo Vespucio, 49, E-41092 Seville, Spain.

出版信息

Carbohydr Res. 2007 Feb 26;342(3-4):448-59. doi: 10.1016/j.carres.2006.11.018. Epub 2006 Nov 21.

DOI:10.1016/j.carres.2006.11.018

PMID:17173881

Abstract

A novel platform for anticancer vaccines has been prepared using glyconanotechnology recently developed in our laboratory. Ten different multifunctional gold glyconanoparticles incorporating sialylTn and Lewis(y) antigens, T-cell helper peptides (TT) and glucose in well defined average proportions and with differing density have been synthesised in one step and characterised using NMR and TEM. Size and nature of the linker were crucial to control kinetics of S-Au bond formation and to achieve the desired ligand ratio on the gold clusters. The technology presented here opens the way for tailoring polyvalent anticancer vaccines candidates and drug delivery carriers with defined average chemical composition.

摘要

最近我们实验室利用糖纳米技术制备了一种新型抗癌疫苗平台。已一步合成了十种不同的多功能金糖纳米颗粒，它们包含唾液酸Tn和Lewis(y)抗原、T细胞辅助肽（TT）以及葡萄糖，平均比例明确且密度不同，并使用核磁共振（NMR）和透射电子显微镜（TEM）进行了表征。连接子的大小和性质对于控制S-Au键形成的动力学以及在金簇上实现所需的配体比例至关重要。这里介绍的技术为定制具有明确平均化学成分的多价抗癌疫苗候选物和药物递送载体开辟了道路。