Toll样受体9（TLR9）及其mRNA在扁平苔藓皮损中的表达
Expression of TLR9 and its mRNA in the lesions of lichen planus.
作者信息
Li Jiawen, Chen Jing, Tan Zhijian, Liu Houjun, Liu Zhixiang
机构信息
Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
出版信息
J Huazhong Univ Sci Technolog Med Sci. 2007 Apr;27(2):203-5. doi: 10.1007/s11596-007-0226-7.
To investigate the role of toll-like receptor 9 (TLR9) in the pathogenesis of lichen planus, the expressions of TLR9 and its mRNA in the lesional skin of lichen planus were detected by immunohistochemical technique (SP) and RT-PCR. As control, normal skin of healthy volunteers was also tested. The immunohistochemical study showed that the expression of TLR9 in the lesional skin of lichen planus was significantly higher than that in the normal controls. The results of RT-PCR showed that both skin lesions and normal controls had TLR9 expression. In skin lesions, the expression level of TLR9 mRNA was 1.6075+/-0.0930, which was significantly higher than that in normal controls (P<0.001). These findings indicated that up-regulated expression of TLR9 and its mRNA might be involved in the pathogenesis of lichen planus.
为探讨Toll样受体9（TLR9）在扁平苔藓发病机制中的作用，采用免疫组化技术（SP法）和逆转录聚合酶链反应（RT-PCR）检测扁平苔藓皮损中TLR9及其mRNA的表达。作为对照，也检测了健康志愿者的正常皮肤。免疫组化研究显示，扁平苔藓皮损中TLR9的表达明显高于正常对照。RT-PCR结果显示，皮肤病变组织和正常对照均有TLR9表达。在皮肤病变组织中，TLR9 mRNA的表达水平为1.6075±0.0930，明显高于正常对照（P<0.001）。这些结果表明，TLR9及其mRNA表达上调可能参与了扁平苔藓的发病机制。
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