阳离子通道阻滞剂（呋塞米）对铜绿假单胞菌PAO1生物膜结构的影响。

The impact of a cation channel blocker (furosemide) on Pseudomonas aeruginosa PAO1 biofilm architecture.

作者信息

Cross Jenny L, Ramadan Hassan H, Thomas John G

机构信息

Department of Otolaryngology Head-Neck Surgery, West Virginia University, Morgantown, WV 26506-9200, USA.

出版信息

Otolaryngol Head Neck Surg. 2007 Jul;137(1):21-6. doi: 10.1016/j.otohns.2007.02.022.

DOI:10.1016/j.otohns.2007.02.022

PMID:17599559

Abstract

OBJECTIVE

To determine if furosemide could have a role in inhibition of biofilm formation.

STUDY DESIGN

A prospective study of 72-hour biofilms grown in the Calgary Biofilm Device (CBD) treated with furosemide and controls.

METHODS

Bacteria were treated with furosemide and controls to determine its antiplanktonic properties. PA01 biofilms were treated with serial 2-fold dilutions of furosemide and controls over a 48-hour period at pH concentrations of 7.0, 8.0, and 9.0.

RESULTS

Furosemide had no effect on planktonic PA01. It had minimal effect at pH below 8.0. A pH of 8.0 and 9.0 reduced biofilms 25%. Furosemide 10 mg/mL reduced biofilms 50% at pH 8.0 and 9.0. It inhibited biofilms to < 30% at concentrations of 0.6 to 0.16 mg/mL at pH 8.0 and < 10% at concentrations of 1.25 to 0.3 mg/mL at pH 9.0.

CONCLUSION

Furosemide was found to be nonantiplanktonic, but does appear to destabilize preformed PA01 biofilms at pH-dependent concentrations.

摘要

目的

确定呋塞米是否在抑制生物膜形成中发挥作用。

研究设计

一项前瞻性研究，用呋塞米处理在卡尔加里生物膜装置（CBD）中生长72小时的生物膜，并设对照组。

方法

用呋塞米和对照处理细菌以确定其抗浮游生物特性。在pH值为7.0、8.0和9.0的条件下，用呋塞米和对照的系列2倍稀释液处理PA01生物膜48小时。

结果

呋塞米对浮游的PA01无作用。在pH值低于8.0时作用最小。pH值为8.0和9.0时生物膜减少25%。10mg/mL的呋塞米在pH值为8.0和9.0时使生物膜减少50%。在pH值为8.0时，浓度为0.6至0.16mg/mL时生物膜抑制率<30%；在pH值为9.0时，浓度为1.25至0.3mg/mL时生物膜抑制率<10%。