利用细菌人工染色体构建体将Cre重组酶靶向特定神经元群体。
Targeting Cre recombinase to specific neuron populations with bacterial artificial chromosome constructs.
作者信息
Gong Shiaoching, Doughty Martin, Harbaugh Carroll R, Cummins Alexander, Hatten Mary E, Heintz Nathaniel, Gerfen Charles R
机构信息
Gene Expression Nervous System Atlas Project, Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA.
出版信息
J Neurosci. 2007 Sep 12;27(37):9817-23. doi: 10.1523/JNEUROSCI.2707-07.2007.
Abstract
Transgenic mouse lines are characterized with Cre recombinase driven by promoters of CNS-specific genes using bacterial artificial chromosome (BAC) constructs. BAC-Cre constructs for 10 genes (, , , , , , , , , and ) produced 14 lines with Cre expression in specific neuronal and glial populations in the brain. These Cre driver lines add functional utility to the >500 BAC-EGFP (enhanced green fluorescent protein) transgenic mouse lines that are part of the Gene Expression Nervous System Atlas Project.
摘要
转基因小鼠品系的特征是使用细菌人工染色体（BAC）构建体，由中枢神经系统特异性基因的启动子驱动Cre重组酶。针对10个基因（，，，，，，，，，和）构建的BAC-Cre构建体产生了14个品系，这些品系在大脑中的特定神经元和神经胶质细胞群体中表达Cre。这些Cre驱动品系为作为基因表达神经系统图谱项目一部分的500多个BAC-EGFP（增强型绿色荧光蛋白）转基因小鼠品系增添了功能实用性。
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