快速控制顶复门寄生虫刚地弓形虫中的蛋白质水平。
Rapid control of protein level in the apicomplexan Toxoplasma gondii.
作者信息
Herm-Götz Angelika, Agop-Nersesian Carolina, Münter Sylvia, Grimley Joshua S, Wandless Thomas J, Frischknecht Friedrich, Meissner Markus
机构信息
Hygieneinstitut, Department of Parasitology, University Hospital Heidelberg, Im Neuenheimer Feld 324, D-69120 Heidelberg, Germany.
出版信息
Nat Methods. 2007 Dec;4(12):1003-5. doi: 10.1038/nmeth1134. Epub 2007 Nov 11.
Abstract
Analysis of gene function in apicomplexan parasites is limited by the absence of reverse genetic tools that allow easy and rapid modulation of protein levels. The fusion of a ligand-controlled destabilization domain (ddFKBP) to a protein of interest enables rapid and reversible protein stabilization in T. gondii. This allows an efficient functional analysis of proteins that have a dual role during host cell invasion and/or intracellular growth of the parasite.
摘要
顶复门寄生虫中基因功能的分析受到缺乏反向遗传工具的限制，这些工具可实现蛋白质水平的轻松快速调节。将配体控制的去稳定化结构域（ddFKBP）与目标蛋白融合，能够在弓形虫中实现蛋白质的快速可逆稳定化。这使得对在寄生虫宿主细胞入侵和/或细胞内生长过程中具有双重作用的蛋白质进行高效功能分析成为可能。
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