高效液相色谱法同时测定猪耳皮肤各层中苯甲酰基苯甲酸-3、视黄醇和醋酸视黄酯的含量。

Simultaneous determination of benzophenone-3, retinol and retinyl acetate in pig ear skin layers by high-performance liquid chromatography.

作者信息

Padula C, Campana N, Santi P

机构信息

Dipartimento Farmaceutico, Università degli Studi di Parma, Viale G.P. Usberti 27/a, 43100 Parma, Italy.

出版信息

Biomed Chromatogr. 2008 Oct;22(10):1060-5. doi: 10.1002/bmc.1024.

DOI:10.1002/bmc.1024

PMID:18521968

Abstract

A new and simple HPLC method was developed and validated for the simultaneous determination of benzophenone-3, retinol and retinyl acetate in pig ear skin layers and percutaneous penetration samples after in vitro permeation experiments. HPLC analysis was performed utilizing a NovaPak C18 column with acetonitrile-water-acetic acid as mobile phase. UV detection was at 325 nm and the run time was 25 min. The detector response was found to be accurate, precise and linear across the analytical range. Analyte extraction from skin layers was done with methanol from the stratum corneum and epidermis, and with acetone from the dermis. Recovery was in all cases better than 90%. The HPLC assay and extraction procedure proposed are simple, rapid, sensitive and accurate. The method was then applied for the determination of benzophenone-3, retinol and retinyl acetate in pig ear skin layers after topical application.

摘要

建立了一种新的简单高效液相色谱法，并对其进行了验证，用于同时测定猪耳皮肤层和体外渗透实验后经皮渗透样品中的二苯甲酮-3、视黄醇和醋酸视黄酯。采用NovaPak C18柱，以乙腈-水-乙酸为流动相进行高效液相色谱分析。紫外检测波长为325nm，运行时间为25分钟。发现在整个分析范围内，检测器响应准确、精密且呈线性。从角质层和表皮中用甲醇提取皮肤层中的分析物，从真皮中用丙酮提取。所有情况下回收率均优于90%。所提出的高效液相色谱测定法和提取方法简单、快速、灵敏且准确。然后将该方法应用于局部应用后猪耳皮肤层中二苯甲酮-3、视黄醇和醋酸视黄酯的测定。