从牛奶中筛选牛凝固酶阴性葡萄球菌的超抗原编码基因。
Screening of bovine coagulase-negative staphylococci from milk for superantigen-encoding genes.
作者信息
Nemati M, Hermans K, Vancraeynest D, De Vliegher S, Sampimon O C, Baele M, De Graef E M, Pasmans F, Haesebrouck F
机构信息
Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
出版信息
Vet Rec. 2008;163(25):740-3.
A collection of 102 coagulase-negative staphylococci (CNS), isolated from cases of subclinical and clinical bovine mastitis and belonging to 10 different species, were screened by PCR for the presence of genes encoding enterotoxins and enterotoxin-like toxins (sea, seb, sec, sed, see, seg, seh, sei, sej, selk, sell, selm, seln, selo, selp, selq and selu), toxic shock syndrome toxin-1 (tst), and exfoliative toxins A and B (eta and etb). No toxin gene sequences were amplified from any of the isolates, indicating that superantigens encoded by genes detectable by the PCR tests used were not involved in the development of subclinical and clinical mastitis in cattle infected with the CNS isolates tested.
从亚临床和临床型牛乳腺炎病例中分离出102株凝固酶阴性葡萄球菌（CNS），它们分属于10个不同的种，采用聚合酶链反应（PCR）对其编码肠毒素和类肠毒素（sea、seb、sec、sed、see、seg、seh、sei、sej、selk、sell、selm、seln、selo、selp、selq和selu）、中毒性休克综合征毒素-1（tst）以及剥脱毒素A和B（eta和etb）的基因进行筛查。未从任何分离株中扩增出毒素基因序列，这表明在所检测的感染CNS分离株的牛中，由所用PCR检测方法可检测到的基因所编码的超抗原不参与亚临床和临床乳腺炎的发生。
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