一种源自2-(吡啶-2-基)苯并咪唑的具有比率传感潜力的锌离子荧光传感器。
A Zn2+ fluorescent sensor derived from 2-(pyridin-2-yl)benzoimidazole with ratiometric sensing potential.
作者信息
Liu Zhipeng, Zhang Changli, Li Yunling, Wu Zhengyi, Qian Fang, Yang Xiaoliang, He Weijiang, Gao Xiang, Guo Zijian
机构信息
State Key Laboratory of Coordination Chemistry, Coordination Chemsitry Institute, School of Chemistry and Chemical Engineering, Nanjing Universitry, Nanjing 210093, PR China.
出版信息
Org Lett. 2009 Feb 19;11(4):795-8. doi: 10.1021/ol802537c.
A fluorescent Zn(2+) sensor based on the 2-(2'-pyridinyl)benzoimidazole (2-PBI) fluorophore has been devised by incorporation with a Zn(2+) ionophore, bis(pyridin-2-ylmethyl)amine. The sensor (PBITA) demonstrates a Zn(2+)-specific emission shift and enhancement with a 1:1 binding ratio. Due to the Zn(2+)-induced coplanation of 2-PBI via reversion/rotation, PBITA is shown to behave as a ratiometric sensor. The intracellular Zn(2+) imaging ability of the sensor has been tested in HeLa cells using a confocal microscope.
通过与锌离子载体双（吡啶 - 2 - 基甲基）胺结合，设计了一种基于2 - （2'-吡啶基）苯并咪唑（2 - PBI）荧光团的荧光锌离子传感器。该传感器（PBITA）表现出锌离子特异性的发射位移和增强，结合比为1:1。由于锌离子通过反转/旋转诱导2 - PBI共平面，PBITA被证明是一种比率传感器。使用共聚焦显微镜在HeLa细胞中测试了该传感器的细胞内锌离子成像能力。
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