营养液中暴露于砷和铬的番茄植株中应激相关基因的表达
Expression of stress-related genes in tomato plants exposed to arsenic and chromium in nutrient solution.
作者信息
Goupil Pascale, Souguir Dalila, Ferjani Ezzedine, Faure Olivier, Hitmi Adnane, Ledoigt Gérard
机构信息
Laboratoire de Physiologie et Génétique végétales, ERTAC-UMR INRA, Université Blaise Pascal-Clermont II, Campus universitaire des Cézeaux, 24, avenue des Landais, 63177 Aubière cedex, France.
出版信息
J Plant Physiol. 2009 Sep 1;166(13):1446-52. doi: 10.1016/j.jplph.2009.01.015. Epub 2009 Apr 17.
The molecular responses of hydroponically cultivated tomato plants to As(V) or Cr(VI) were assessed by transcript accumulation analysis of genes coding for products potentially involved in heavy metal tolerance. A quantitative real-time PCR experiment was performed with Hsp90-1, MT2- and GR1-like protein genes using RNA isolated from tomato roots or shoots treated for 24h with As(V) or Cr(VI) at concentrations ranging from 80 to 640 microM. Both transient metallic treatments induced Hsp90-1 transcript accumulation in tomato plants. MT2- and GR1-like transcripts accumulated in tomato roots treated with As(V) but were only slightly affected by Cr(VI) treatment. Tomatoes showed phenotypic symptoms to heavy metal toxicity when plants were exposed to Cr(VI) but not As(V). Plant lethality was observed at 1280 microM Cr(VI), indicating that tomatoes were more tolerant to As than Cr stress under the experimental conditions used here.
通过对可能参与重金属耐受性的产物编码基因进行转录积累分析，评估水培番茄植株对砷（V）或铬（VI）的分子反应。使用从经80至640微摩尔浓度的砷（V）或铬（VI）处理24小时的番茄根或茎中分离的RNA，对Hsp90 - 1、MT2和类GR1蛋白基因进行了定量实时PCR实验。两种短暂的金属处理均诱导番茄植株中Hsp90 - 1转录本积累。MT2和类GR1转录本在经砷（V）处理的番茄根中积累，但受铬（VI）处理的影响较小。当植株暴露于铬（VI）而非砷（V）时，番茄表现出重金属毒性的表型症状。在1280微摩尔铬（VI）时观察到植株致死，表明在此处使用的实验条件下，番茄对砷胁迫的耐受性高于铬胁迫。
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