乙酰肝素酶-1在胃癌侵袭中的作用。
Role of heparanase-1 in gastric carcinoma invasion.
作者信息
Yingying Xu, Yong Zhang, Zhenning Wang, Xue Zhang, Li Jiang, Yang Luo, Huimian Xu
机构信息
Department of Surgical Oncology and General Surgery, the First Hospital of China Medical University, Shenyang, P.R. China.
出版信息
Asian Pac J Cancer Prev. 2009 Jan-Mar;10(1):151-4.
PURPOSE
The heparan sulfate-degrading endoglycosidase may mediate tumor invasion and metastasis. It is known that heparanase-1 (HPA-1) plays an important role in cleaving heparan sulfate. In this study we investigated its potential role in gastric carcinoma malignant behaviour.
METHODS
To assess the role of HPA-1, we suppressed its expression using small interfering RNA (siRNA). The human heparanase-1 specific siRNA was transfected to the human gastric carcinoma cell line SGC7901. The effect of gene silencing was assessed by Real-Time PCR and Western Blot. The invasion potential of the cells was evaluated using an in vitro cell invasion model system.
RESULTS
The HPA-1 specific siRNA significantly suppressed expression of heparanase-1 in SGC7901 cells. Invasion after RNAi treatment through membranes in a model system was significantly decreased.
CONCLUSION
HPA-1 facilitates gastric carcinoma invasion by up-regulating endoglycosidase activity in tumor cells.
目的
硫酸乙酰肝素降解内切糖苷酶可能介导肿瘤侵袭和转移。已知乙酰肝素酶-1（HPA-1）在切割硫酸乙酰肝素中起重要作用。在本研究中，我们调查了其在胃癌恶性行为中的潜在作用。
方法
为评估HPA-1的作用，我们使用小干扰RNA（siRNA）抑制其表达。将人乙酰肝素酶-1特异性siRNA转染至人胃癌细胞系SGC7901。通过实时聚合酶链反应（Real-Time PCR）和蛋白质免疫印迹法（Western Blot）评估基因沉默的效果。使用体外细胞侵袭模型系统评估细胞的侵袭潜能。
结果
HPA-1特异性siRNA显著抑制SGC7901细胞中乙酰肝素酶-1的表达。在模型系统中，RNA干扰处理后细胞穿过膜的侵袭能力显著降低。
结论
HPA-1通过上调肿瘤细胞中的内切糖苷酶活性促进胃癌侵袭。
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