核苷酸切除修复交叉互补基因 2（ERCC2）、核苷酸切除修复交叉互补基因 1（ERCC1）多态性与单倍型、烹调油烟与中国不吸烟女性肺腺癌风险
ERCC2, ERCC1 polymorphisms and haplotypes, cooking oil fume and lung adenocarcinoma risk in Chinese non-smoking females.
机构信息
Department of Epidemiology, School of Public Health, China Medical University, Shenyang 110001, PR China.
出版信息
J Exp Clin Cancer Res. 2009 Dec 14;28(1):153. doi: 10.1186/1756-9966-28-153.
BACKGROUND
Excision repair cross-complementing group 1 (ERCC1) and group 2 (ERCC2) proteins play important roles in the repair of DNA damage and adducts. Single nucleotide polymorphisms (SNPs) of DNA repair genes are suspected to influence the risk of lung cancer. This study aimed to investigate the association between the ERCC2 751, 312 and ERCC1 118 polymorphisms and the risk of lung adenocarcinoma in Chinese non-smoking females.
METHODS
A hospital-based case-control study of 285 patients and 285 matched controls was conducted. Information concerning demographic and risk factors was obtained for each case and control by a trained interviewer. After informed consent was obtained, each person donated 10 ml blood for biomarker testing. Three polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.
RESULTS
This study showed that the individuals with the combined ERCC2 751AC/CC genotypes were at an increased risk for lung adenocarcinoma compared with those carrying the AA genotype [adjusted odds ratios (OR) 1.64, 95% confidence interval (CI) 1.06-2.52]. The stratified analysis suggested that increased risk associated with ERCC2 751 variant genotypes (AC/CC) was more pronounced in individuals without exposure to cooking oil fume (OR 1.98, 95%CI 1.18-3.32) and those without exposure to fuel smoke (OR 2.47, 95%CI 1.46-4.18). Haplotype analysis showed that the A-G-T and C-G-C haplotypes were associated with increased risk of lung adenocarcinoma among non-smoking females (ORs were 1.43 and 2.28, 95%CIs were 1.07-1.91 and 1.34-3.89, respectively).
CONCLUSION
ERCC2 751 polymorphism may be a genetic risk modifier for lung adenocarcinoma in non-smoking females in China.
背景
切除修复交叉互补基因 1（ERCC1）和基因 2（ERCC2）蛋白在 DNA 损伤和加合物的修复中发挥重要作用。DNA 修复基因的单核苷酸多态性（SNP）被怀疑会影响肺癌的风险。本研究旨在探讨 ERCC2 751、312 和 ERCC1 118 多态性与中国非吸烟女性肺腺癌风险之间的关系。
方法
采用病例对照研究，对 285 例患者和 285 例匹配对照进行了研究。由经过培训的访谈者向每位病例和对照收集人口统计学和危险因素信息。在获得知情同意后，每人捐献 10 毫升血液用于生物标志物检测。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法确定了 3 种多态性。
结果
本研究表明，与携带 AA 基因型的个体相比，携带 ERCC2 751AC/CC 基因型的个体患肺腺癌的风险增加[校正比值比（OR）1.64，95%置信区间（CI）1.06-2.52]。分层分析表明，在未接触烹调油烟（OR 1.98，95%CI 1.18-3.32）和未接触燃料烟雾（OR 2.47，95%CI 1.46-4.18）的个体中，与 ERCC2 751 变异基因型（AC/CC）相关的风险增加更为明显。单体型分析显示，A-G-T 和 C-G-C 单体型与非吸烟女性肺腺癌风险增加相关（OR 分别为 1.43 和 2.28，95%CI 分别为 1.07-1.91 和 1.34-3.89）。
结论
ERCC2 751 多态性可能是中国非吸烟女性肺腺癌的遗传风险修饰因子。
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